ABSTRACT
In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.
Subject(s)
Animals , Cattle , Female , Antibodies, Protozoan/blood , Cattle Diseases/diagnosis , Coccidiosis/diagnosis , Diagnostic Tests, Routine/methods , Immunoassay/methods , Neospora/immunology , Staining and Labeling/methods , Veterinary Medicine/methodsABSTRACT
By immunizing mice with killed whole bacterial cells of Brucella abortus S [99], a panel of six hybridomas producing monoclonal antibodies [mAb] specific for the surface antigens of this bacterium were produced. ELISA was used to screen the hybridoma supernatants. Immunoblots of the cell extract indicated that three mAb were specific for S-LPS [Ba-1, Ba-2, Ba-3] and three others were reactive with major outer membrane proteins [OMP] [Ba-4, Ba-5, Ba-6]. The OMP recognized by these antibodies were the proteins with molecular masses of 25-27 kDa [Ba-4, Ba-5] and 36-38 kDa [Ba-6]. None of the four mAb including Ba-3, Ba-4, Ba-5 and Ba-6 cross reacted with any other bacteria close to Brucella abortus, but Ba-1 and Ba-2 cross reacted with B. melitensis 16M and B. suis. By using cell extract and killed whole cell Ag in ELISA, it was indicated that all mAb except Ba-6 have better reactivity with cell extract Ag, but Ba-6 mAb reacted with killed whole cell Ag better than cell extract Ag